the effect of silver nanoparticles, thyme and savoury extracts injection to egg on the hatchability, digestive and immunity parameters at hatchability

an experiment was conducted with 855 fertilized eggs in 8 treatments (injection in 5th day of incubation) and 11 treatments (injection in 17th day of incubation) with 3 replicates of 15 eggs each. treatments included first control (no injection), second control (injection of 0.5 ml. of sodium chloride); in 5th day, experimental treatments included 30 and 45 mg. silver nanoparticles, and 100 mg. of thyme and savoury. in 17th day treatments included 30, 45, and 60 mg. silver nanoparticles, and 100, 150, and 175 mg. of thyme and savoury extracts. injection in 5th day indicated that the bursa weight reached its maximum in treatments 75 and 100 mg. of thyme, and 75 mg. of savoury, and in treatments with 30 and 45 mg. of silver nanoparticles had a minimum level‎ (p<0.05) ‎. the spleen weight was maximum in treatments of 75 mg. of thyme and was minimum in 30 mg injection. of silver nanoparticles ‎ (p<0.05) ‎. hatching-day shell weight was minimum in control, 30, and 45 mg. of silver nanoparticle, which was significant compared to other treatments (thyme and savoury) ‎ (p<0.05). alkaline phosphatase was significantly higher in silver nanoparticles injected eggs compared to other treatments ‎ (p<0.05). in 17th day, significantly higher ratio of yolk sac weight to body weight was observed in treatments 150 and 175 mg. of savoury compared to that in second control ‎(p<0.05)‎. cholesterol levels were significantly higher in treatments 175 mg of thyme and 100 and 150 mg. of savoury than those in control (p<0.05). in conclusion injection of nanosilver improved bone parameters in broilers. in addition, by ‎injection of thyme and savoury extract in fertilized eggs, the anti-oxidant activity of glucose in ‎these extracts and other properties of herbs could be used effectively.